Fig. 6

Analysis of what RNA-seq reads could not be mapped to the gene model. The left pie chart indicates where RNA-seq reads mapped to in the genome. The exon (orange), intron (gray), and up/down-stream (yellow) are ones that mapped to the predicted gene model. The other transcripts (dark blue) represents unmapped reads to the gene model but mapped to the genome. The unmapped to genome represents unmapped reads to the genome (light blue). The right pie chart represents proportion between not used reads (singleton) and used reads (contigs) in RNA assembly using reads of other transcripts. The bottom left figure shows result of GSEA analysis, BP (blue) shows biological process, CC (red) shows celluar component, and MF (green) shows molecular function