Fig. 1

Bolitoglossa ramosi. a Wild adult salamander of B. ramosi (7–10 cm snout to tail) where the forelimbs had completed 28 weeks of regeneration. b Tissues used during this analysis. The control limb was the intact limb of the animal; the white line indicates the point of the amputation. The regenerative tissues were the blastema of 20 days post-amputation (dpa), blastema of 40 dpa, blastema 60 dpa, and early palette of 70 dpa