Table 2 Primers used for gene expression studies by qPCR analysis
Gene description | Target gene | Primers | Primer sequence (5′ → 3′) |
|---|---|---|---|
Anti-Müllerian hormone | amh | Fw | CAGTCACTCTCTGCAGCCTTACAA |
Rv | CAACATTGAATCTCCATTTCAGTTTAC | ||
Probe | TTTGCCCTCGGGTTGCTTTCCTGTT | ||
Calreticulin precursor | calr | Fw | ACGCCGCTGACTCTACCATCT |
Rv | TCACATCATCTGAGACCAGGAAGTT | ||
Catenin beta-1 | ctnnb1 | Fw | CCCCAGGCGACAGCAAT |
Rv | CCAAATCACAATGCAGGTTGA | ||
Cryptochrome-1 | cry1 | Fw | AGGTCAGCCGGCCAACA |
Rv | AACGGCTCAGAGTCACACTCAAA | ||
Endoplasmin precursor | hsp90b1 | Fw | TGAAGCGGATTTGGTTTATTGG |
Rv | CGCCGTCAATGTCAAGTTCAT | ||
Eukaryotic 18S ribosomal RNA | 18S | TaqMana | proprietary information |
Follicle stimulating hormone subunit beta | fshb | Fw | TCACGGAGGCATCACCATCA |
Rv | GCTCTTGGCAACGGGTATGA | ||
Probe | ACCTGCGCCGGCCTGTGC | ||
Frizzled-8 precursor | fzd8l | Fw | GGAGCGACCCATTATTTTCCT |
Rv | CCGGCAATTAGTCTGACAATGTAA | ||
G1/S-specific cyclin-D1 | ccnd1 | Fw | AAGCAAATCCTGTGCAAGCA |
Rv | CGGAGGGTTGGCAATGAA | ||
Gamma-crystallin M2 | crygm2 | Fw | CCACCTTCTACGAGGACAGGAA |
Rv | TGGCACCTGCTCATGTAGGA | ||
Gap junction alpha-1 protein | gja1 | Fw | CCTAAGGGCTCTCTTCTTACTTCTGA |
Rv | CACTCCAGTCACCCATGATGAA | ||
Geminin | gmnn | Fw | TGCAAGTCCTCCAGCAAGCT |
Rv | CCCTTGTTCTGCATTCCACTGT | ||
Gonadotropin releasing hormone receptor 4 | gnrhr4 | Fw | TCAACCCACTGGCGATCAAT |
Rv | CGTGATGGTCACACTGTGGAATA | ||
Probe | AGTGTGATTCTGTCTGTTCCCCAGATGCTG | ||
Gonadotropin subunit beta-2 | lhb | Fw | ACACTGCCCATCAGGACACAA |
Rv | GGGAGATCAAGGTGCCTACATG | ||
Growth-regulated alpha protein-like | LOC106577834 | Fw | TCATCATGAATACTGCAATGACTGTT |
Rv | TATCCTCTCCCTTGTATCATGCAGAT | ||
Insulin-like 3 (Leydig cell) | insl3 | Fw | CTCCGGAGCTTGGACAACAC |
Rv | AGTCCTCAGGTTGGCAAATTGAT | ||
Insulin-like growth factor 3 | igf3 | Fw | GACCGACCGACAAGATGCA |
Rv | TGCAAGGCACAATATGGAGTACA | ||
Noggin | nog | Fw | GATGGAGGTGCCTGCAGAGA |
Rv | TTCAGTTCGAGCAGGAGCATTT | ||
Olfactomedin-like protein 2B precursor | olfml2b | Fw | CGAAGGGCAACGGAAATGTA |
Rv | ATCCAGTTGTACGGCAGCTTGT | ||
Prostaglandin E synthase 3 | ptges3 | Fw | CCGTGGCTGAGGCTAACAAA |
Rv | CCTCCCAGTCTTTCCAGTTGTTAA | ||
Testin | tes | Fw | CGTGTCTGAAATGCAAGGACAA |
Rv | GTGGTCCGTAAGCCCACACTT | ||
Vasotocin-neurophysin VT 2 precursor | neu4 | Fw | GGACACTGCGCTGCAACA |
Rv | GTACCGACTCTCCCCCAAACA | ||
Wnt-11 precursor | wnt11l | Fw | CCCCAGGCCAGCACACTA |
Rv | ACCACCTGCAGAGCCTCACT |