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Fig. 3 | BMC Genomics

Fig. 3

From: Comprehensive assessment of multiple biases in small RNA sequencing reveals significant differences in the performance of widely used methods

Fig. 3

Accuracy Assessment. a Individual points represent the absolute difference of each synthetic miRNA quantification from the mean of all quantifications of the equimolar synthetic sequences for each small RNA sequencing method. b The variance of all the quantification estimates for the synthetic sequences. c The percent variance of synthetic sequence quantifications explained by each of these sequence characteristics: GC content, length, free energy of the predicted secondary structure (FoldG), identity of the first (5′) and last (3′) two bases, the count of individual bases, and the presence of repeat sequences, such as duplets of the same base or quadruplets of the same base. The heatmap legend shows the percentage of variance from 0 to 10%. d The percent variance explained by each of the sequence characteristics but weighted by the overall variance of each method, as shown in b. The heatmap legend shows the percentage of variance from 0 to 10%

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