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Fig. 4 | BMC Genomics

Fig. 4

From: Integration of CLIP experiments of RNA-binding proteins: a novel approach to predict context-dependent splicing factors from transcriptomic data

Fig. 4

Overview of pipeline to predict splicing factors using CLIP and splicing. A toy example with a cassette exon is shown. 1) Selecting splicing regions: The cassette exon has two isoforms which give rise to Path 1 (p1), Path 2 (p2) and reference (pref). Splicing regions (typically 300–400 nt upstream and down- stream the AS events [26]) are represented in orange. 2) The ExS matrix (Events x Splicing factors) is built by mapping RBPs against the splicing regions. 3) The Percent Spliced-In (PSI) of all the events is estimated from RNA-seq or microarrays and a Fisher’s exact test enrichment is performed to get a ranking of RBPs according CLIP binding sites

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