Table 1 The qRT-PCR primer sequences of TSGs in the pig
Gene | Primer sequences (5′-3′) | Sizes (bp) |
|---|---|---|
SYCP3-F | CATCGAAGCGAACACCTCAG | 150 |
SYCP3-R | GACATCCTCCTCTGAACCACTC | |
SLC26A8 -F | TTGCATCTCTGGTAAGCGCA | 121 |
SLC26A8 -R | AGGTAGGGTAGGACGTTGCT | |
SYCE3-F | AATCTCAGTGCAGGCCACG | 121 |
SYCE3-R | CTCCATCTCCTCCTTGCAGT | |
CRISP2-F | CTGCTGTGCTGCTACCATCT | 138 |
CRISP2-R | TGGCAGGTGGAGAGACTGAT | |
SOX30-F | TCGCTCACCTACCTACTCTGT | 127 |
SOX30-R | AAGTGTGATGGGACTTGGGC | |
DDX4-F | TGGGTGGTTTTGGTGTTGGA | 153 |
DDX4-R | AGCCGCCTCTCTTGGAAAAA | |
ODF1-F | GGAAAGCCATCCGAGCCATA | 176 |
ODF1-R | CACACACACCTTTCCGTCCT | |
FAM71D-F | ACGACATCTGCATCCCAGAC | 183 |
FAM71D-R | AAGAGCAGATGCCCACAGTC | |
β-actin-F | CTCCATCATGAAGTGCGACGT | 114 |
β-actin-R | GTGATCTCCTTCTGCATCCTGTC |