Fig. 5

Workflow for the identification of the origin of the flanking norgDNA of transgenic inserts. a. Sequences of three SunUp transformation plasmid derived inserts with borders and the bwa alignment process. b. A strategy using high-throughput and massive paired-end mapping to identify deletions in Sunset relative to the reference genome. Insertions in SunUp were predicted from paired-end spans larger than a specified cutoff (size of a transgenic insert). c. Histogram plots exhibiting the inner distance of mapped paired-ends in regions of three inserts with borders