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Fig. 5 | BMC Genomics

Fig. 5

From: Genome re-sequencing and reannotation of the Escherichia coli ER2566 strain and transcriptome sequencing under overexpression conditions

Fig. 5

RNA-seq for variant calling under pressure from overexpression. a) The experimental design. Each group (B37, without plasmid; Y37, with pTO-T7 plasmid overexpressed) had three biological replicates. b) Visualization of BAM files of the B37 (left panel) and Y37 (right panel) in the Integrative Genomics Viewer. Based on the reannotation, one mutant was identified at position 1,094,824 C > T, located within the 3′ non-coding region of the transcription factor gene lacI. c) Mutation detected by Sanger sequencing of the B37 and Y37 genomic samples

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BMC Genomics

ISSN: 1471-2164

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