Fig. 6

Expression of cold-responsive genes and physiological responses in different indica rice. a-c Heat maps show the qRT-PCR results of 18 differentially expressed genes in different indica rice varieties. The 2^-dCT values (expression profile) are plotted for upregulated genes (a) downregulated genes (b), and upregulated DREB regulon genes (c) respectively, under control and cold shock conditions. d Histogram representing percent germination of nine different indica rice varieties e, f Histogram representing the data obtained from physiological response experiments for IR64, IR36, Hamsahamas, CB1, and Heera indica rice varieties. d Histogram showing Relative Electrical Conductivity percent (REC%) for the five rice varieties. f Histogram showing the ROS generation expressed as fluorescence units/mg of protein. d-f In each case, the experiment was repeated in triplicate sets. The bar values are expressed as mean ± S.D. (n = 3; Two-tailed paired t-test, the level of significance was represented by * (where *P < 0.05 and > 0.0332, **P < 0.0332 and > 0.0021, ***P < 0.0021 and > 0.0002, ****P < 0.0001). g Images showing germination of different indica varieties post cold stress. Image was taken 7 days post germination