Fig. 2From: Genomic nucleotide-based distance analysis for delimiting old world monkey derived herpes simplex virus speciesEstablishing viral species cutoff value. Pairwise distances in the Old World monkey virus alignment were calculated using Mega 7 [46], and the frequencies plotted using the R package. A kernel density plot was also generated and combined with the distance frequencies (a). A distance cutoff value was established by determining the trough of the kernel plot, which is depicted by a vertical dotted line (8.94%). Mega 7 was used to calculate between group distances which is shown in Figure bBack to article page