Fig. 1

Experimental design and ATAC-seq quality metrics. a Overview of tissues collected from adult male cattle and pigs for ATAC-seq, wherein the Tn5 transposase preferentially cuts DNA at accessible sites and simultaneously inserts sequencing adapters. b Scatterplots showing Pearson correlation of normalized genome-wide ATAC-seq signal (reads per million; RPM) between biological replicates for brain cortex and lung. c Heatmaps depicting normalized ATAC-seq signal at all TSS, sorted by signal intensity. Signal shown for brain cortex and lung. d Normalized ATAC-seq signal in cattle tissues at the GAPDH locus (a housekeeping gene), as well as several genes with tissue-specific activity. Pink bars indicate that signal exceeded the viewing range (up to 20 RPM). e Normalized ATAC-seq signal in pig tissues at the same genes. f PCA of normalized ATAC-seq signal in consensus open chromatin identified in cattle. G) PCA of normalized ATAC-seq signal in consensus open chromatin identified in pig