Fig. 6

Overexpression of PCK1 promotes lipoid accumulation in buffalo intramuscular adipocytes. a Micrographs of EGFP-positive cells under white and fluorescent light in the Ad_EGFP (control) and Ad_PCK1 groups on day o and day 6 of adipogenesis induction. The preadipocytes were induced to differentiation 24 h after adenovirus transduction. Scale bar, 200 μm. b-d, g, h The RNA expression levels of PCK1, PPARG, C/EBPα, FABP4, FAT/CD36, GPAT4, DGAT1, HSL, LPL, and PPARα on day 0 and day6 of adipogenesis induction in buffalo intramuscular adipocytes transfected with Ad_EGFP and Ad_PCK1. Expression level data are presented as the mean ± S.E. (n = 6); * indicates p < 0.05; ** indicates p < 0.01. e Images of Oil Red O staining in buffalo intramuscular adipocytes transfected with Ad_EGFP and Ad_PCK1 on day 6 of adipogenesis induction. Scale bar, 50 μm. f Histogram showing the quantitation of Oil Red O staining by spectrophotometry. N.C., negative control by using isopropanol. Data are presented as the mean ± S.E. (n = 6); * indicates p < 0.05; ** indicates p < 0.01