Fig. 3
From: Illuminating the dark side of the human transcriptome with long read transcript sequencing
Comparing different pipelines on the UHRR Iso-Seq Dataset. a Diagram of workflow for the four pipelines used to analyze the UHRR Iso-Seq dataset. b Example of the Polish pipeline missing the full length transcript model due to low read coverage for the 5′ complete read. Since Cluster/Polish filters out any reads that do not cluster with at least one other read, the single read support for the longer model was filtered out in the Polish pipeline but captured by TAMA. In this case, the truncated model in Polish happens to match a transcript model in the Ensembl annotation