Fig. 5

Error rate estimation and wobble across pipelines. a The average percent of alignment mismatch by mismatch type across pipelines. b Average splice junction wobble across all transcript models which matched the Ensembl annotation in all four pipelines. A splice junction wobble threshold of 30 bp on each side of the splice junction was allowed for matching for these plots. Note that wobble greater than 30 bp is possible due to wobble walking. c Scatter plots to illustrate the amount of wobble across all pipelines assessed on the transcript models used in Average Splice Junction Wobble plot