Fig. 3

EMBR-seq can quantify the transcriptome from low input total RNA. a Similar levels of rRNA depletion and mRNA enrichment are observed when the starting amount of total RNA is decreased from 100 ng to 0.02 ng (n ≥ 2, except at 0.02 ng where n = 1). The control represents average data of control samples made from different input levels of total RNA. The 100 ng data is reproduced from Fig. 2a. b Compared to the control samples, more genes are detected when starting with at least 2 ng input total RNA. Fewer genes are detected when starting total RNA decreases to 0.02 ng. c Gene transcript counts are highly correlated (Pearson r = 0.98) between 100 ng and 20 ng input total RNA in EMBR-seq. Datasets from lower starting total RNA are also well correlated to the 100 ng samples (Additional file 1: Fig. S6)