Skip to main content
Fig. 6 | BMC Genomics

Fig. 6

From: Genomic differences between the new Fusarium oxysporum f. sp. apii (Foa) race 4 on celery, the less virulent Foa races 2 and 3, and the avirulent on celery f. sp. coriandrii

Fig. 6

Homo- (within strain) and hetero-(between strain) conidial anastomosis tubes. a-g Micrographs. F. oxysporum f. sp. apii race 4 (FoaR4) conidia were pre-stained with wheat germ agglutinin that was conjugated to either Alexa Fluor 488 or Alexa Fluor 594 (not shown) and then mixed with differently labeled F. oxysporum f. sp. coriandrii (Foci)3–2 or F. oxysporum f. sp. apii race 2 (FoaR2). After conidia were incubated on polystyrene in conditions conducive for conidial anastomosis tube (CAT) formation, a Leica with epifluorescent GFP and rhodamine filters, and a 63X objective with differential interference contrast optics, was used to record the images. Bars represent 10 μm. Short CATs are indicated with a single arrow and longer CATs are indicated with an arrow at each end of the CAT. a-b FoaR4 and Foci form hetero-CATs. c-g FoaR4, FoaR2 and Foci form homo-CATs. Hetero-CATs between FoaR4 and FoaR2 were never observed. f Three conidia form a CAT cluster. h-m Frequency of formation of homo- and hetero-CATs in mixtures of strains. h-j FoaR4 and Foci. k-m FoaR4 and FoaR2. CATs that could be categorized as hetero- or homo-CATs were recorded, as were the numbers of each of the labeled conidia in the same field of view. For each field of view in which there was at least one score-able CAT, the normalized frequency of the CAT category was computed to adjust for the number of possible pairs of each type. Data from three independent trials with similar results are shown here; a total of 741 and 695 CATs were evaluated in (h-j) and (k-m), respectively. A non-parametric Kruskall-Wallis test indicates that normalized frequencies of homo-Foa race 4, homo-Foci, and hetero-CATs of Foa race 4 and Foci were statistically indistinguishable (P = 0.41). In contrast, Foa race 4 did not form CATs with Foa race 2

Back to article page