Fig. 3
From: Efficient COI barcoding using high throughput single-end 400 bp sequencing
Phylogenetic tree of 660 successful barcodes of moth, with outgroup Drosophila melanogaster. The red circle reveals samples containing fungi COI barcode, and blue for Wolbachia COI barcode. We obtained the taxonomic information of each sample according to that of its best hits on the BOLD database and it may suffer misidentification due to inaccurate records on the database. The phylogeny tree revealed that some specimens could be wrongly identified based on an inadequate database in specific linage. For example, the best hit of #035 in Plate #4 (green arrow) with 100% similarity in BOLD database belongs to Crambidae family, however, the second hit with 99.85% similarity belongs to Erebidae family. This type of incorrect placement is prone to occur among early-release records, which suggests a new record of specimen need to be carefully reviewed when add to a database, also indicating that morphological identification is still important