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Fig. 2 | BMC Genomics

Fig. 2

From: Long non-coding RNA exploration for mesenchymal stem cell characterisation

Fig. 2

Overview of annotated genes and unannotated transcripts enriched in BM-MSCs. a Left pannels represented: i/ Ensembl v90 transcript categories and distribution, ii/ transcripts distribution expressed in MSCs, showing unnatotated transcripts obtained with ab initio reconstruction by StringTie vs annotated transcripts (expression >0.1 TPM), iii/ predicted lncRNAs from unnanotated reconstructed transcripts include new lncRNAs with intergenic (Mlinc) and antisens (Mlncoa) RNA categories. b-c-d Distribution of transcript length, exon length and GC percentage across different categories respectively with the same colors as in a pannel: coding transcripts (blue), annotated lincRNA (pink), annotated overlapping antisens lncRNA (purple), novel lincRNA (Mlincs, yellow), novel overlapping antisens RNA (Mloanc, red). e Representation of annotated genes (top pannel) and unannotated transcripts (bottom pannel) overexpresed in MSC versus non-MSC types (log2FC >0.5 and padj <0.05), separately showed in MA plot. f Total number of transcripts by category. The colored bar indicated the number of differentially expressed annotated genes (Ensembl v90) and unannotated transcripts (Mlinc and Mloanc). gGlobal expression in BM-MSCs (with Sleuth normalisation) of the same categories as in f for annotated genes and unannotated transcripts

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