Fig. 4

RNase-H based depletion method affected RNA quality. a Coverage summary plots among the four protocols. The probe hybridization method covered the entire gene body uniformly. However, the enzymatic removal method revealed skewing to 3′ region of genes. The gene body coverage plot shows samples shown as dotted lines, normalized genomic position on the horizontal axis (5′ to 3′ region of genes) and average coverage on the vertical axis. b Representative screenshot in the long transcript between two different depletion methods. GLOBINClear Kit covered more reads in the middle of the gene than Ribo-Zero Plus Kit. From exon 11 to 19 of the ATM gene were visualized on the IGV. NEBgr, NEBNext® Globin & rRNA Depletion Kit; RZr, Ribo-Zero Plus rRNA Depletion Kit; GZr, Globin-Zero Gold rRNA Removal Kit