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Fig. 2 | BMC Genomics

Fig. 2

From: Unbiased RNA-Seq-driven identification and validation of reference genes for quantitative RT-PCR analyses of pooled cancer exosomes

Fig. 2

Gene expression levels and stability of candidate reference genes for exosomes predicted with RNA-Seq data. Expression levels of ten candidate genes sorted by their respective expression levels (a). Expression levels of ten candidate reference genes (blue bars) compared with those of nine commonly used housekeeping genes (green bars) (b). Expression stability of candidate reference genes as measured by the coefficient of variation (“CV”) (c). Comparison of “CV” values between candidate reference genes and classical housekeeping genes (p = 8.10e-04, Wilcoxon rank-sum test) (d). Expression stability of candidate reference genes as measured by the average expression stability value (“M”) (e). Comparison of “M” values between candidate reference genes and classical housekeeping genes (p = 0.0279, Wilcoxon rank-sum test) (f)

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