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Fig. 5 | BMC Genomics

Fig. 5

From: Systematic comparison of high-throughput single-cell RNA-seq methods for immune cell profiling

Fig. 5

Differentially-expressed (DE) gene detection: a Fold change (FC) versus false discovery rate (FDR) calculated using a hurdle model (MAST) for mouse genes in EL4 vs IVA12 cells. Shown is a representative subsample of mouse cells (n=199) using the 10x 3′ v2 method demonstrating the criteria for declaring DE genes (FDR < 10− 4); DE genes are highlighted in red. b Number of significant DE genes calculated using MAST between EL4 and IVA12 cells by method. Error bars represent the 95% confidence interval. The total number of significant DE genes are plotted in red, the number of DE genes with > 1.5-fold difference in expression in bulk RNA-seq (5868 genes) are plotted in cyan. c Median bulk RNA-seq expression (FPKM) of all significant DE genes (red) or DE genes with > 1.5-fold difference (cyan). Error bars represent 95% confidence interval

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