Fig. 6
From: Systematic comparison of high-throughput single-cell RNA-seq methods for immune cell profiling
Cell recovery by cell identifier (CID) thresholding: a Example of using the transposed log-log empirical cumulative density plot of the total counts of each CID to identify cell-containing droplets . Common thresholding points, the ‘knee’ and the ‘inflection’ are indicated with arrows. The knee is the point at which the signed curvature is minimized, the inflection is the point at which the first derivative is minimized. b The fraction of cells above the knee or inflection are plotted. c Fraction of cells below mitochondrial rate threshold (listed in Supplement Table 4) relative to knee point. Samples are colored by cell sample mixture listed in Supplement Table 2