Fig. 3

Taxol biosynthesis in callus cells and tissues Taxol biosynthesis was significantly upregulated in callus cells. a Taxol biosynthesis pathways. Genes/pathways in red indicated they were upregulated, blues were downregulated, darks had no differences. And bold gens mean they were targeted by miRNAs. MEP and MVA are short for Non-mevalonate pathway and Mevalonate pathway. Solid arrows mean the enzymatic step were certificated, while dotted arrows mean there were several unknown steps. b Taxanes content in callus cells and tissues. DBIII: 10-Deacetylbaccatin III, BIII: baccatin III, EDT: 10-deacetyl taxol. c Box-plot of expression values of taxol biosynthesis genes. Ns mean the different was not significantly in callus and tissues. TS (TASY): taxadiene synthase, DBAT: 10-deacetylbaccatin III-10-O-acetyl transferaseferase, PAM: phenylalanine ammonia-lyase, T5H: taxadiene 5-alpha hydroxylase, TAT: taxadienol acetyl transferase, T10H: 5-alpha-taxadienol-10-beta-hydroxylase, T13H: 13-alpha-hydroxylase gene, DBBT: taxane 2-alpha-O-benzoyltransferase, DBTNBT: 3′-N-debenzoyltaxol N-benzoyltransferase, BAPT: phenylpropanoyltransferase