Fig. 5
From: A transcriptional landscape of 28 porcine tissues obtained by super deepSAGE sequencing
Luciferase reporter assay of the RUNX1 targets. One wild-type promoter construct (containing the predicted RUNX1 binding site), two mutant constructs (mutated or not containing the binding site) were investigated. The mutant construct (black) was identical to the wild-type, except that the RUNX1 binding site was deleted or mutated. The line graphs show the luciferase activity after the reporter plasmids were transfected into PBMCs (a-c) or macrophages (d-f). Three RUNX1 target genes have been investigated (a and d: TLR-2, b and e: LCK, c and f: VAV1). The error bars represent the mean ± standard deviation of three duplicate sample sets