Skip to main content
Fig. 6 | BMC Genomics

Fig. 6

From: CATS: Cas9-assisted tag switching. A high-throughput method for exchanging genomic peptide tags in yeast

Fig. 6

G418 resistance does not segregate correctly following meiosis in strains that retained their GFP signal. Two of the G418+ strains that retained their GFP signal (NUP170- and BIR1-tag) were mated with a wild-type unlabeled strain to form diploids alongside one mixed RFP/GFP strain (MPS1-tag) and one strain that had successfully converted to RFP (HTB2-RFP). The diploids were sporulated and tetrads were dissected on YPD, each tetrad was horizontally arranged and replica-plated to -HIS and YPD G418 media, then scored for growth as indicated in Supplementary Table 4. Images show the subsequent colony formation of replicated spore colonies

Back to article page

BMC Genomics

ISSN: 1471-2164

Contact us