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Fig. 7 | BMC Genomics

Fig. 7

From: CATS: Cas9-assisted tag switching. A high-throughput method for exchanging genomic peptide tags in yeast

Fig. 7

Conversion to different fluorescent tags using variants of the template plasmid. The strain encoding Htb2-GFP was tested with 4 different template construct plasmids, specified for each horizontal panel, each encoding a different fluorescent tag combined with ADH1 promoter-driven Kanamycin. Strains were then checked with fluorescence microscopy, and signals in the DIC, RFP, Azurite, CFP and YFP channels are shown. Scale bars represent 5 μm. GFP appears in both the CFP and YFP channels due to the use of a single band pass filter. It remains distinguishable from the CFP and YFP signals however as each of these appear in only one channel

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