Fig. 2

Optimization of number of partitions and coverage for the Supernova assembler. Different number of partitions were randomly selected using the partition (GEM) barcodes while also varying the number of reads per partition to optimize the coverage. These were provided as input for the assembler. For each resulting assembly the NG50 length and LG50 count were calculated with genome size assumed to be 320 Mb [23]. NG50 value is the scaffold/contig length at which half of the genome (~ 160 Mb) is contained in scaffolds/contigs at or above that length. LG50 is the number of contigs needed to reach N50. Arrow heads indicate optimized parameters