Fig. 6

Principle component analysis (PCA) of 1100 most variable genes among the 4 metamorphotic stages. Gene expression (transcripts per million) was calculated for each of the stages; egg, larvae, pupae, and adult using RSEM [94]. A coefficient of variation was determined for each gene and used to determine the most variable genes. Eigenvector coordinates for the stages (egg, larvae, pupae, and adult) on the first 2 components are shown in red. Coordinates of the individual genes on the first 2 principle components (circle of correlation) are shown as black dots