Whole-genome sequencing of Puccinia striiformis f. sp. tritici mutant isolates identifies avirulence gene candidates

Table 1 Numbers and percentages of heterozygous and homozygous of EMS-induced SNPs in mutant isolates of Puccinia striiformis f. sp. tritici detected by mapping to the primary scaffolds of isolate 11–281

Mutant	No. of SNPs	Heterozygous		Homozygous
isolates	No. of SNPs	No.	Percent (%)^a	No.	Percent (%)^b
M11-Yr36–1	117,035	109,070	91.84	3726	8.16
M11-Yr21	115,281	103,417	71.05	17,707	28.95
M11-YrTr1	113,504	99,896	97.78	208	2.22
M11-Yr24–1	112,757	98,611	93.91	5463	6.09
M11-Yr9–2	109,247	100,435	97.94	201	2.06
M11-Yr17	100,118	92,493	99.07	227	0.93
M11-Yr39	89,676	84,213	71.65	17,350	28.35
M11-Yr10	86,237	80,478	93.54	5508	6.46
M11-YrA+	86,114	80,365	97.74	223	2.26
M11-Yr9–1	85,723	80,208	93.57	5515	6.43
M11-Fielder	85,258	79,750	87.45	14,146	12.55
M11-Yr6	85,195	79,658	88.01	13,608	11.99
M11-Yr9–4	72,356	61,995	71.52	17,402	28.48
M11-Yr1–2	71,929	60,913	92.49	3470	7.51
M11-Yr1–3	62,064	53,728	93.32	5749	6.68
M11-YrSP-1	61,507	44,014	91.93	8812	8.07
M11-Yr2–1	61,352	43,571	98.12	191	1.88
M11-Yr76–2	61,243	43,742	71.02	17,781	28.98
M11-YrExp2	61,198	43,848	70.92	17,722	29.08
M11-Yr2–2	61,171	43,464	85.68	10,361	14.32
M11-Paha	61,094	43,692	86.57	8336	13.43
M11-Yr36–2	60,944	43,222	93.32	5759	6.68
M11-YrSP-2	60,943	43,717	71.42	17,501	28.58
M11-Yr76–1	46,186	42,716	89.71	11,864	10.29
M11-Yr76–3	45,671	41,945	93.19	7965	6.81
M11-Yr43	24,453	24,226	92.38	7625	7.62
M11-Yr44	10,139	9948	71.56	17,493	28.44
M11-Yr1–1	9881	9658	71.73	17,226	28.27
M11-Yr8	9768	9567	84.68	11,016	15.32
M11-Yr31	9353	9145	93.50	5537	6.50
Average	67,913	58,724	86.47	9190	13.53

^aThe percentage of heterozygous SNPs was calculated as the number of heterozygous SNPs divided by the total number of SNPs of each isolate times 100
^bThe percentage of heterozygous SNPs was calculated as the number of homozygous SNPs divided by the total number of SNPs of each isolate times 100

ISSN: 1471-2164