Fig. 2
From: Multiple freeze-thaw cycles lead to a loss of consistency in poly(A)-enriched RNA sequencing
Higher Noise in Samples with More Freeze-Thaw Cycles. From left to right, noise—the randomness in read counts between technical replicates—is estimated using Euclidean distance, RMSE, Pearson correlation, and Spearman correlation. a Box plots of noise for samples that underwent either one or two freeze-thaws. b A linear regression was used to determine the expected noise without freeze-thaw (N0, pink) and the expected change in noise with each additional freeze-thaw (ΔN, green). All estimates are significant (p ≤ 0.05)