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Table 2 Primers used in the amplification of AB and CD fragments from L. monocytogenes EGDe genomic DNA

From: High-pressure processing-induced transcriptome response during recovery of Listeria monocytogenes

Primers

Sequence

5′- 3′

Amplimer obtained

lmo1013_AB_fwd

TCGAATTCGAAGCTTCTGCATAACACCAATAGTCGCCCCT

Upstream flanking fragment (AB) of lmo1013 coding region including the start codon (ATG)

lmo1013_AB_rev

AGCGAATTGGCGTCTTTTTACATTTTTTGGTCCACATCCT

lmo1013_CD_fwd

AGGATGTGGACCAAAAAATGTAAAAAGACGCCAATTCGCT

Downstream flanking fragment (CD) of the lmo1013 coding region including the stop codon (TAA)

lmo1013_CD_rev

ATGACGTCGACGCGTCTGCATAGTGCAGTTATTACGATTG

lmo2229_AB_fwd

TCGAATTCGAAGCTTCTGCATCAGGTGGCTCGATTGCAAA

Upstream flanking fragment (AB) of the lmo2229 coding region including the start codon (ATG)

lmo2229_AB_rev

GTAAAATGTGCTTTTAATTACATGTAACTCTCCTATCTTC

lmo2229_CD_fwd

GAAGATAGGAGAGTTACATGTAATTAAAAGCACATTTTAC

Downstream flanking fragment (CD) of the lmo2229 coding region including the stop codon (TAA)

lmo2229_CD_rev

ATGACGTCGACGCGTCTGCATTCACCATCTAAAGTAATTT