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Fig. 2 | BMC Genomics

Fig. 2

From: Impact of PNPase on the transcriptome of Rhodobacter sphaeroides and its cooperation with RNase III and RNase E

Fig. 2

The pnp mutant and the wild type strain differ in growth behaviour, pigmentation as well as in growth under different temperatures and under organic peroxide stress. a Schematic overview of the pnp operon. In the pnp mutant, the KH-S1 domains were deleted and substituted with a gentamicin resistance gene. A stop codon was inserted at the end of the remaining pnp coding region. Upper panels show the RNA read coverage in the wild type and pnp mutant strain. b The pnp mutant grows slower than the wild type under microaerobic cultivation and does not reach the wild type optical density during stationary phase when cultivated under phototrophic conditions. Red: wild type; blue: pnp mutant; n = 3. c, d Exponentially growing pnp mutant cultures exhibit reduced carotenoid and bacteriochlorophyll a (Bchl a) concentrations under microaerobic conditions in comparison to the wild type strain. Phototrophically cultivated, the pigment concentrations are increased in the mutant. The p-values were calculated using two-sided Student’s t-test (*: p < 0.05; n.s.: not significant). e On solid malate minimal agar, the growth of the pnp mutant strain is strongly impaired when the plates are incubated at 4 °C or 42 °C. The organic peroxide tBOOH (300 μM final concentration) diminishes growth of the wild type but prevents growth of the pnp mutant strain. Biological triplicates are shown for each growth condition

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