Fig. 5

The PNPase mutation leads to a change in RNA 3′ end composition. a Histogram illustrating the different distribution sizes of all 1072 differential 3′ ends. Q₁ = 1 nt; Q₂ = 1 nt; Q₃ = 3 nt; arithmetic mean = 3.86 nt. b Distribution of the log₂-fold changes of all differential 3′ ends, comparing the pnp mutant strain and the wild type. 82% of the 3′ ends are enriched in the mutant. c All 3′ ends were assigned to genes or UTRs according to their genomic position and then grouped in feature classes. Most 3′ ends in CDS (coding sequences) and UTRs were enriched in the pnp mutant, but more tRNA 3′ ends were detected in the wild type. d + e The boxplots show the absolute number of 3′ ends enriched in the mutant per gene d and the relative number (normalized to the gene length) (e). Overall 306 genes were observed to harbour at least one 3′ end enriched in the mutant. Every dot represents one gene