Fig. 4

Time resolved secretion dynamics of proteins in saliva of uninfected and Borrelia burgdorferi (Bb) infected. The normalized spectral abundance factor as the index for relative protein abundance was graphed using PRISM 8 for five functional protein categories of uninfected (blue line) and Bb infected (red line) tick saliva during feeding. (AA) represent serine protease inhibitors (serpin) secreted by Bb infected saliva only, (AB) represent serpins secreted by both uninfected and Bb infected ticks, (B) = non-serpin protease inhibitors; (CA) represent M12 proteases, (CB) represent other metalloproteases, and (CC) represent other proteases, (D) represent cholesterol and lipid binding proteins, (E) represent antimicrobial peptides, and (F) represent histamine binding proteins/ lipocalins. Please note that the table insert that is included in the figure lists accession numbers and putative annotations. The X-axis data points represent unfed stage, feeding time points every 12 h through 72 h, and replete-fed ticks. The Y-axis represents relative abundance as indicated by average NSAF values from the three LC-MS/MS runs