Fig. 1

The minimum number of nanopore reads required to generate an accurate consensus sequence. Four HCV full length amplicons were sequenced with both Illumina (Miseq) and nanopore platforms. Consensus sequences made from randomly picked nanopore reads (in multiples of 100, each read > 8500 nt) were compared against the consensus sequence made from the entire volume of Illumina reads which had an average coverage of 17,000 nt per position (used here as the gold standard). Each data point demonstrates mean pairwise mismatches and standard deviation. The accuracy does not improve further beyond 300 nanopore reads