Fig. 2

Accuracy of nanopore sequence output in reproducing high and low frequency variants in a mix of sequences. Plasmids with Hepatitis C virus E1E2 inserts (1800 nt) were mixed in different proportions (0.1–93%) with 5 plasmids per mix and approximately 15 such mixes. Each mix were tagged with the same nanopore barcode and sequenced on the same flow cell. The original proportions of each insert could be reproduced post-sequencing even when the input frequency was as low as 0.1%. The original plasmid insert sequence was used as a reference to identify corresponding nanopore reads. X axis- input plasmid frequency calculated as a % based on concentration, Y-axis output frequency calculated as the number of nanopore reads per HCV insert as a % of the total nanopore reads per mix