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Fig. 3 | BMC Genomics

Fig. 3

From: Adaptation of Oxford Nanopore technology for hepatitis C whole genome sequencing and identification of within-host viral variants

Fig. 3

Accuracy of pooling multiple samples with PCR based barcoding for nanopore sequencing on the same flow cell. 52 full-length HCV amplicons isolated from different patients were sequenced concurrently on Nanopore (with PCR based barcoding) and Illumina platforms and pairwise mismatches were compared across consensus sequences. For samples with a high number of mismatches, either nanopore or Illumina sequence did not have an adequate coverage in some segments of the genome (adequate coverage was defined as > 300 reads for nanopore and > 100 reads for Illumina)

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