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Fig. 2 | BMC Genomics

Fig. 2

From: Massively parallel gene expression variation measurement of a synonymous codon library

Fig. 2

Validation of protein distribution reconstructed from Sort-seq. a Distributions of single-cell fluorescence as measured by flow cytometry for six randomly isolated library members. b Sort-seq-reconstructed single-cell fluorescence (pink columns) and the fitted curves (black) to a Gamma distribution from three independent Sort-seq experiment (from top the bottom) for the same six library isolates as shown in (a). c The correlation on mean fluorescence measured from Sort-seq and flow cytometry for another sixteen randomly isolated library members. d Mean GFP fluorescence and CV2 for all 219 library members passing our filters. Error bars represent standard deviation across the three experiments. The six isolated library members shown in (a) and (b) are highlighted in purple

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