Fig. 6From: Comparative transcriptome analysis of the newly discovered insect vector of the pine wood nematode in China, revealing putative genes related to host plant adaptationValidation of sequencing data by qRT-PCR. a The expression changes for each candidate gene as measured by RNA-seq and qRT-PCR. b Scatter plot showing correlation between log2(fold change) obtained via RNA-seq and qRT-PCR. Log2(fold change) qPCR was calculated by −∆∆Ct = − [(CtPt – Ctnom) – (CtPk – Ctnom)]Back to article page