From: Consistent ultra-long DNA sequencing with automated slow pipetting
DNA isolation | Method | Pores available at run start | Number of bases (Gb) | Number of reads | Longest read (bp) | N50 size (bp) | N90 size (bp) |
---|---|---|---|---|---|---|---|
1 | Aspiration | 1505 | 4.25 | 661,189 | 737,462 | 21,327 | 2212 |
Aspiration | 1528 | 3.92 | 644,261 | 645,672 | 19,617 | 2077 | |
Rotation | 1432 | 2.56 | 283,481 | 862,322 | 25,482 | 3818 | |
Rotation | 1362 | 4.32 | 365,489 | 782,247 | 30,174 | 4891 | |
Automation | 1452 | 4.97 | 795,794 | 1,021,308 | 13,082 | 2333 | |
Automation | 1442 | 4.92 | 496,674 | 1,253,204 | 29,063 | 3933 | |
Mean | 1454 | 4.16 | 541,148 | 883,703 | 23,124 | 3211 | |
2 | Aspiration | 883 | 3.34 | 673,876 | 682,744 | 12,695 | 1909 |
Aspiration | 1093 | 3.16 | 598,310 | 774,556 | 13,510 | 2030 | |
Rotation | 1046 | 5.4 | 617,128 | 508,355 | 17,107 | 3407 | |
Rotation | 1208 | 7.41 | 637,349 | 526,752 | 24,163 | 4800 | |
Automation | 1182 | 7.21 | 1,209,053 | 508,355 | 12,382 | 2342 | |
Automation | 1571 | 10.92 | 1,608,258 | 526,752 | 14,589 | 2654 | |
Mean | 1164 | 6.24 | 890,662 | 587,919 | 15,741 | 2857 | |
3 | Aspiration | 1420 | 5.06 | 883,721 | 605,755 | 11,809 | 2127 |
Aspiration | 1629 | 6.46 | 755,277 | 798,240 | 18,470 | 3381 | |
Rotation | 1311 | 8.02 | 915,877 | 661,883 | 16,458 | 3566 | |
Rotation | 818 | 4.24 | 386,642 | 584,414 | 19,942 | 4285 | |
Automation | 1421 | 4.97 | 596,368 | 602,104 | 21,358 | 3539 | |
Automation | 1410 | 6.89 | 545,627 | 717,975 | 31,810 | 5864 | |
Mean | 1335 | 5.94 | 680,585 | 661,729 | 19,975 | 3794 |