Fig. 3

Validation of the SNP-based analysis. a Heat map of Pearson’s correlation coefficient between the stigma / pollen transcripts from the SNP-based analysis and transcript information from previously published data. b Number of stigma or pollen (preferentially / specifically) -expressed genes at C0 selected by nFPKM (Additional file 3: Table S4). c RT-PCR and sequence analysis of stigma or pollen specifically-expressed genes at C0. Genes are selected among the top 20 specifically-expressed genes; their rank according to their expression level are presented. RNAs were extracted from pollinated stigmas at C0 and we analyzed their SNP-information by RT-PCR and sequencing. SNP number corresponds to the number of SNPs present in the sequenced regions. d Top ten GO term enrichment categories (biological processes) of stigma or pollen preferentially-expressed genes at C0. Enrichment analysis was performed with the one thousand top expressed genes in stigma (left) or pollen (right), respectively. Selection criteria for genes analysed in c (sex-specific), and d (sex-preferential), are described in text