Fig. 2

Model based clustering of time course expression profiles for differentially expressed genes. a All genes found to have significantly different response patterns by ImpulseDE2 in any of the different comparative analyses were clustered using the R package MBCluster-seq. The clustering is organized in the heatmap such that the rows are the individual conditions at each time point and the columns are the individual genes. The rows are grouped by Genotype x Treatment (GxT) condition ordered from 0 h (bottom) to 4 h (top) of refeeding after starvation. The grayscale of each column is the log-fold change of the normalized expression data standardized to the zero sum mean for a gene. Each expression pattern cluster has been associated with a given color and number for reference. b Mean values are plotted for expression across all genes within each cluster at each time point. Line color is used to identify the represented cluster