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Fig. 5 | BMC Genomics

Fig. 5

From: A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeast

Fig. 5

Inferring fitness effects from guide RNA abundance changes. a, b Consistent rate of exponential decay in abundance for guides targeting a SUI3, which encodes eIF2β, and b STV1 during pooled, competitive growth. Two distinct barcodes are shown for each guide in two replicate cultures. c As in (a) and (b), showing the constant or slightly increasing abundance for three distinct barcodes linked to no guide RNA. d Barcode abundance changes across replicate cultures. Fitness estimates of associated guide RNAs are shown as well. e Enrichment of strong negative fitness effects in guides targeting essential genes. Guide-level fitness estimates are shown for all unambiguous promoters, as well as classification according to essentiality. Barcode-level analysis is shown for all barcodes linked to non-targeting guides. f Most essential genes have at least one strongly deleterious guide. The most negative fitness effect across all guides is shown for genes with unambiguous promoters. The cumulative distribution of barcode fitness effects is shown for non-targeting barcodes

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