Fig. 3
From: Transcriptional profiling of identified neurons in leech
In situ hybridization (ISH) verification of expression patterns found by RNASeq. Eight Trinity genes from four clusters were chosen to represent the widest variety of potential staining patterns. a-h. In each panel: the graph at left denotes the expression levels of a Trinity gene in the RNASeq analysis, with the cell type on the X-axis and the average normalized read count of the transcript in transcripts per kilobase million (TPM) on the Y-axis; error bars denote the standard error of the mean; the micrograph at right shows a typical ISH staining pattern for the Trinity gene in an adult H. verbana (Hve) ganglion. All ganglia are oriented ventral-side up unless otherwise indicated. Tph = tryptophan hydroxylase, ddc = dopa decarboxylase, pcad1 = protocadherin 1, hcn4 = hyperpolarization-activated cyclic nucleotide-gated channel 4, vgkc1 = voltage-gated potassium channel 1, cola = collagen-alpha, ip3rb2 = inositol triphosphate receptor b2, hyp1 = hypothetical 1