Fig. 6
From: Transcriptional profiling of identified neurons in leech
Expression and molecular phylogeny of leech HCN genes. a. Expression levels in four cell types and the remainder of the ganglion of the 5 HCN genes represented in our Hirudo transcriptome. Error bars denote the standard error of the mean. TPM = Transcripts per Kilobase Million. b. A maximum-likelihood phylogeny showing the evolutionary context of the eight HCN genes identified in the Helobdella robusta genome (red). Bootstrap values are shown adjacent to each branch; scale bar = the average number of substitutions per site along each branch. Vertebrate = a clade consisting of 11 vertebrate sequences (see Fig. S3), Urochordate = a clade consisting of Ciona intestinalis and Ciona savignyi, Leech = Helobdella robusta, Limpet = Lottia gigantea, Sea Hare = Aplysia californica, Squid = Doryteuthis pealeii, Oyster = Crassostrea gigas, Brachiopod = Lingula anatina, Fly = Drosophila melanogaster, Mosquito = Aedes aegypti, Silkmoth = Bombyx mori, Bee = Apis mellifera, Lobster = Panuliris argus, Sea Urchin = Strongylocentrotus purpuratus, Human = Homo sapiens