Fig. 5
Effects of ribosome biosynthesis and cell cycle inhibitors on fiber cell development. a Box plots indicating overall gene expression change (z-score) in ribosome protein subunit genes and cell cycle related genes (G1-M). Box colors indicate samples of epidermal at 0 DPA (blue), epidermal at 2 DPA (red), and fiber at 2 DPA (black) of GhTM-1 (dark colored), Gh3–79 (intermediate colored), and GhMD17 (light colored). Three asterisks indicate a statistical significance level of P < 0.001 (Student t-test). b Bar plots indicate gene expression levels (FPKM) of TOR, RAPTOR, LST8, and EBP1 in 2 DPA samples. Colors are identical to those in (a). One, two, and three asterisks indicate statistical significance levels of P < 0.05, < 0.01, and < 0.001, respectively (Student t-test). c Total area (mm2) of ovules of GhTM-1 and GhMD17 incubated in culture for 21 days with control (grey), Rbin-1 (blue), and Roscovitine (red) treatments. Three asterisks indicate a statistical significance level of P < 0.001 (Student’s t test). d Photos of typical ovules with fibers in GhTM-1 and GhMD17 ovules (insets, right bottom) incubated with mock (control, left), Rbin-1 (middle), and Roscovitine (right). Bars = 10 mm