Identification of polycistronic transcriptional units and non-canonical introns in green algal chloroplasts based on long-read RNA sequencing data

Table 1 General characteristics of C. lentillifera chloroplast genome and comparison with other Caulerpa species

Species	Accession Number ^a	Length (kb)	Overall GC content (%)	No. of genes			ORFs^c	Reference
Species	Accession Number ^a	Length (kb)	Overall GC content (%)	Protein coding genes ^b	rRNA genes	tRNA genes	ORFs^c	Reference
C. lentillifera	This study	126.97	32.43	76	3	28	39	This study
C. lentillifera	MG753774.1	119.40	32.68	76 (75)	3	28	45	[20]
C. manorensis	NC_037367.1	140.60	34.62	76 (75)	3	28	36	[13]
C. cliftonii	NC_031368.1	131.14	37.64	76 (74)^d	3	27	34	[12]
C. racemosa	NC_032042.1	176.52	33.64	76 (75)	3	27	34	[21]
C. okamurae	KX809677.1	148.27	34.54	76 (73)^e	3	27	28	–
C. verticillata	NC_039523.1	148.11	33.52	76 (75)^e	3	28	57	[11]

^a Accession numbers refer to sequences in Genbank database at NCBI
^b The numbers outside and inside parentheses represent corrected and previously reported gene numbers, respectively. tilS was included for counting; fragmented genes (such as rpoBa and rpoBb) were counted once
^c Open reading frames (ORFs) > = 300 bp were included in the count; Previously annotated ORFs that related to tilS were excluded. For data consistency, the ORFs of C. racemosa (NC_032042.1) and C. lentillifera (MG753774.1) were reannotated
^d The mis-annotated petL in previous report was included in corrected result of C. cliftonii
^e The miss annotated rpl32 and petL in previous report were included in corrected result of C. okamurae and C. verticillata

ISSN: 1471-2164