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Fig. 6 | BMC Genomics

Fig. 6

From: The effect of methanol fixation on single-cell RNA sequencing data

Fig. 6

PCA using transcripts with different length and GC contents. (A) PCA performed using different transcripts sets. In each plot, transcripts are selected based on lengths and GC contents thresholds. Transcripts selected were used for analysis and plotting. As transcripts with longer lengths and higher GC are used for PCA, PC1 is gradually dominated by the fixation effect. (B) PC1 loadings of cells in PCAs performed with different transcripts sets. (C) With PCAs performed with increasing lengths and GC contents thresholds, corresponding length or GC content statistic of the top 500 transcripts from PC1s was plotted

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