Fig. 4

Maximum-likelihood (ML) based phylogenetic reconstructions of Diabrotica virgifera virgifera gene families with members putatively involved in Bacillus thuringiensis and chemical insecticide resistance. A ATP binding cassette (ABC) transporter protein superfamily. Clades corresponding to subfamilies A to H are indicated with respective colors as defined by included Tribolium castaneum orthologs (bold). B Cytochrome P450 (CYP) gene families: Color coded members are indicated as defined by T. castaneum orthologs (bold). Node support of ≥ 500 from 1,000 bootstrap iterations are indicated by dot size on a 10-point scale and shown numerically for nodes separating subfamilies. Internal scale indicates number of amino acid changes per site from among retained positions within corresponding amino acid alignments