Fig. 2

Peaksat package estimates sequence depth required to reach saturation for ChIP-seq. A Meta-pool saturation curves that show the number of peaks (thousands, k) versus the number of reads (millions, M) for the meta-pool of H4K5ac and H4K8ac ChIP-seq libraries. B Target depth estimation procedure. Blue and red lines indicate data from constituent replicates to the meta-pool. Solid-dark lines are observed data from the initial low-depth sequencing run. Dotted lines are linearly extrapolated from the initial sequencing and were used to predict the number of reads required to reach saturation. Solid-light lines are observed data from combined initial and follow-up sequencing runs. C Tables used to calculate the number of additional reads required to reach saturation (Needed) based on the number of reads initially sequenced (Initial) and the estimated total target reads to reach peak saturation (Target). All values are in millions of reads